1)時間:2013/12/10 注:部分請與廠家工程師聯(lián)系
2)樣品:土壤
3)儀器:主機,Tissuelyser植物組織研磨器(上海凈信實業(yè)有限公司生產(chǎn))
4)配件:鋼罐和凈信研磨珠
5)步驟:
A.取土壤樣本,分割成幾小塊,分別放入鋼罐中。
B.每個鋼罐中放入幾顆凈信研磨珠,分別將鋼罐旋好后,再置入研磨適配器中,將適配器固定在研磨儀中。
C.設(shè)置Tissuelyser全自動組織研磨儀的研磨時間和研磨頻率。啟動研磨器。
D.研磨完全停止后,拿出適配器上的鋼罐,旋下鋼罐蓋子,取出研磨好的土壤樣本樣品。
E.然后取出研磨成細粉的土壤放入離心管中,在離心管中加入微量的裂解液,用于下一步實驗。
6)結(jié)果:
由圖可以清晰的看出,研磨后的土壤很均勻的細粉狀,說明用Tissuelyser全自動組織研磨儀研磨土壤效果非常良好,可以用于后續(xù)的實驗操作。
上海凈信的全自動樣品快速研磨儀處理與手工液氮研磨處理后提取 FT 的 DNA、RNA 在濃度、純度上均無顯著性差異 。但手工研磨有較大的樣本損失率,對于DNA提取濃度較低的樣品來說,自動研磨儀可以做到樣品零損失,在DNA提取上有著明顯的優(yōu)勢,且手工處理的過程復(fù)雜、易交叉污染,操作所用時間等方面均不及上海凈信的全自動樣品快速研磨儀,因此自動研磨儀對于DNA提取工作相對于手工研磨處理有很大優(yōu)勢。
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