提取動物關節(jié)軟骨組織總RNA
實驗目的
研磨破碎軟骨組織(大鼠膝關節(jié)軟骨),提取其總RNA。
實驗原理
充分磨碎軟骨樣品(大鼠膝關節(jié)軟骨),再用相關試劑提取其總RNA。
實驗材料和器具
樣品:大鼠膝關節(jié)軟骨
儀器:多樣品組織研磨儀(上海凈信,Tissuelyser-24)
耗材:研磨罐,研磨珠
試劑:液氮若干
實驗步驟
需了解具體步驟,請致電廠家
實驗結果
采用本實驗方法提取的動物關節(jié)軟骨組織總RNA的收率高,并且純度高、質量、完整性好,可以滿足分子生物學研究應用。
總RNA甲醛變性膠電泳結果
Ⅱ型膠原PCR擴增產(chǎn)物電泳結果
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