實驗目的
對大腸桿菌進行破壁,提取其蛋白質。
實驗原理
通過研磨珠的撞擊,破碎大腸桿菌的細胞壁,使其內容物流出,進而提取其蛋白質。
實驗材料和器具
樣品:大腸桿菌
儀器:全自動樣品冷凍研磨儀(上海凈信,JXFSTPRP-96)
耗材:離心管,研磨珠(上海凈信)
實驗步驟
需了解具體步驟,請致電廠家
實驗結果
圖注:泳道3:marker
泳道2:0號對照
泳道1:含8M尿素上清
泳道0:bingding buffer 上清
泳道 0 1 2 3
本蛋白形成包涵體,在使用此研磨機后進行上樣電泳。圖譜清晰,說明本研磨機適用于含包涵體菌體的破碎。
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