實(shí)驗(yàn)?zāi)康?/strong>
對(duì)大量和小量酵母菌分別進(jìn)行研磨,進(jìn)而提取其核酸或蛋白。
實(shí)驗(yàn)原理
通過(guò)研磨珠的撞擊,充分破碎酵母菌細(xì)胞壁,使其內(nèi)容物流出,進(jìn)而提取目標(biāo)成分。
實(shí)驗(yàn)材料和器具
樣品:酵母菌
儀器:全自動(dòng)樣品冷凍研磨儀(上海凈信,Tissuelyser-24)
耗材:離心管,不銹鋼研磨珠(上海凈信,中號(hào)研磨珠)
實(shí)驗(yàn)步驟
需了解具體步驟,請(qǐng)致電廠(chǎng)家
實(shí)驗(yàn)注意事項(xiàng)
1.少量酵母菌研磨好后,實(shí)驗(yàn)室需要檢驗(yàn)離心管中酵母是否研磨達(dá)到要求,可以采用重疊離心管離心方法:具體步驟為,取一管研磨好裝有酵母的離心管,在底部用針或者其他尖銳東西扎一小孔,再在該離心管底部套一新離心管,一起離心。最后將新離心管中得到的酵母液進(jìn)行鏡檢,若鏡檢無(wú)完整酵母細(xì)胞,則研磨效果達(dá)到要求。
2.如果上述步驟檢測(cè)效果不理想,可以在第一步裝樣本前,先把酵母樣本放入離心機(jī),稍微離心,去除上浮液,再繼續(xù)實(shí)驗(yàn)。
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