實驗目的:研磨腎組織,更有效快速的提取核酸蛋白。
實驗地點:浙江大學紫荊港校區(qū)醫(yī)學院C座214實驗室
實驗材料和器具:
樣品:小鼠腎組織
儀器:高通量組織研磨儀(上海凈信,JXFSTPRP-24)
耗材:專用研磨管(上海凈信),研磨珠(上海凈信)
試劑:PBS緩沖液
實驗步驟
1.準備好研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的腎組織樣品剪一小塊,放入準備好的研磨管中,加入研磨珠,蓋好蓋子;放到適配器中,適配器可以事先放在冰箱先預冷,在干磨過程中相對的控制溫度。
3.將適配器裝入凈信研磨儀中;
4.設定好參數,啟動儀器至研磨程序結束。
實驗結果
Previous:多樣品組織研磨儀研磨破碎皮膚、動植物及各材料標本實驗-上海凈信
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