實(shí)驗(yàn)?zāi)康?/strong>
將牛骨磨成粉末后在種植細(xì)胞觀察細(xì)胞的活性及生長。
實(shí)驗(yàn)地點(diǎn):中南大學(xué)湘雅醫(yī)學(xué)院孝
實(shí)驗(yàn)材料和器具
樣品:牛的骨頭
儀器:全自動液氮冷凍研磨儀(上海凈信,JXFSTPRP-24)
耗材:專用適配器一套,研磨管一對
試劑:液氮若干
實(shí)驗(yàn)步驟
1.準(zhǔn)備好不銹鋼研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的樣品直接放入準(zhǔn)備好的研磨管中,不需要剪短,加入一顆研磨珠。蓋好蓋子;
3.將裝有組織樣品的研磨管放入液氮中,冷凍至不沸騰為止;
4.取出已經(jīng)冷凍的適配器,把冷凍好的不銹鋼研磨管取出插入適配器中;
5.將適配器裝入凈信研磨儀中;
6.設(shè)定好參數(shù),啟動儀器至研磨程序結(jié)束,可得到粉末狀樣品。
需了解具體步驟,請致電廠家
實(shí)驗(yàn)結(jié)果
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