研磨心肌組織實(shí)驗(yàn)?zāi)康模?/strong>
研磨心肌組織,更有效快速的提取核酸蛋白。
實(shí)驗(yàn)地點(diǎn):中南大學(xué)湘雅醫(yī)學(xué)院孝騫樓護(hù)理實(shí)驗(yàn)室461
實(shí)驗(yàn)材料和器具:
樣品:小鼠心肌組織
儀器:全自動(dòng)樣品快速研磨儀(上海凈信,JXFSTPRP-24)
耗材:專用研磨管(上海凈信),研磨珠(上海凈信)
試劑:PBS緩沖液
研磨心肌組織實(shí)驗(yàn)步驟:
1.準(zhǔn)備好研磨管,將研磨管和研磨球清洗干凈,并滅菌滅酶;
2.將需要研磨的心肌組織樣品剪一塊,放入準(zhǔn)備好的研磨管中,加入幾顆研磨珠,蓋好蓋子;放到適配器中,適配器可以事先放在冰箱先預(yù)冷,在干磨過(guò)程中相對(duì)的控制溫度。
3.將適配器裝入凈信研磨儀中;
4.設(shè)定好參數(shù),啟動(dòng)儀器至研磨程序結(jié)束。
研磨心肌組織實(shí)驗(yàn)結(jié)果:
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