實驗目的:實驗室里的老師需要用到研磨設備,來研磨植物組織提DNA(比如柑橘、柿子之類),偶爾需要研磨一些其他組織,由于之前沒有接觸過我們的這類產(chǎn)品,我們便和老師們一起做了此次的研磨實驗。
實驗地點:武漢大學
實驗材料和器具:
樣品:生柿子
工具:上海凈信全自動樣品研磨儀(Tissuelyser-24)
耗材:離心管,研磨珠(鋼珠)
實驗步驟:
1.準備好適配器,鋼珠
2.將裝好的樣品規(guī)范放入Tissuelyser-24研磨儀中
批量研磨樣品,一般情況下,由于客戶研磨的是生柿子,較難處理,纖維較多,果肉較硬每個研磨管放若干顆4mm鋼珠,設置頻率,若需要研磨更細致可以加鋼珠
3.得到如圖所示研磨樣品
注意:
1、每支離心管樣品量一般不超過放心管體積的三分之二。
2、樣品盡量對稱放置,以保證研磨時的受力平衡。
實驗過程、結(jié)果圖示:
上海凈信Tissuelyser-24
如圖為我們的實驗材料,研磨的是生柿子
實用儀器介紹:
此款儀器為上海凈信實業(yè)發(fā)展有限公司生產(chǎn)的全新全自動樣品研磨儀是一種特殊的、快速的、高效率的、多試管的一致系統(tǒng)。它采用了特殊的垂直上下一體震動模式,通過研磨珠(氧化鋯、鋼珠、玻璃珠、陶瓷珠)的高頻往復振動、撞擊、剪切??焖俚膶崿F(xiàn)目的。使研磨的樣品具有更加充分、更均勻、樣品重復性更好、樣品之間沒有交叉污染。
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