直徑35mm玻璃底培養(yǎng)皿,底部帶有500um網(wǎng)格
用于細(xì)胞和細(xì)胞簇的重找回
便于進(jìn)行固定區(qū)域的細(xì)胞計(jì)數(shù)
玻璃蓋可用于TIRF和單細(xì)胞應(yīng)用
應(yīng)用:
重新定位細(xì)胞和細(xì)胞簇,如轉(zhuǎn)染細(xì)胞的克隆挑選
對(duì)某一劃定區(qū)域的細(xì)胞計(jì)數(shù),如計(jì)算轉(zhuǎn)染效率
為細(xì)胞運(yùn)動(dòng)提供一個(gè)參考結(jié)構(gòu)
獨(dú)特的單個(gè)細(xì)胞處理,如顯微注射
在確定的比例尺下追蹤軸突的生長(zhǎng)
貨號(hào):
貨號(hào) | 產(chǎn)品名稱 | 規(guī)格(個(gè)/盒) |
81148 | μ-Dish 35 mm,高壁,玻璃底,50微米格子培養(yǎng)皿 | 30 |
81168 | μ-Dish 35 mm,高壁,玻璃底,500微米格子培養(yǎng)皿 | 30 |
80156 | μ-Dish 35 mm,低壁,ibiTreat底部處理,500微米格子培養(yǎng)皿 | 60 |
80151 | μ-Dish 35 mm,低壁,無包被,500微米格子培養(yǎng)皿 | 60 |
81166 | μ-Dish 35 mm,高壁,ibiTreat底部處理,500微米格子培養(yǎng)皿 | 60 |
81161 | μ-Dish 35 mm,高壁,無包被,500微米格子培養(yǎng)皿 | 60 |
技術(shù)特征:
底部500 μm網(wǎng)格
字母和數(shù)字標(biāo)識(shí)的4 x 400 個(gè)方格(A-U; 1-20)
網(wǎng)格和細(xì)胞在一個(gè)焦面
底部為D 263 M肖特玻璃,厚度為170 μm +/- 10 μm
蓋子有鎖扣設(shè)計(jì),可以減少蒸發(fā)
產(chǎn)品規(guī)格:
Grid repeat distance | 500 μm | Coating area using 400 μl | 4.1 cm2 |
? μ-Dish | 35 mm | ? observation area | 21 mm |
Volume | 2 ml | Height with / without lid | 14/12 mm |
Growth area | 3.5 cm2 | ||
底部 | Glass coverslip No. 1.5, selected quality, 170 μm +/- 10 μm |
Optical properties | ||
Refractive index (nD 589 nm) | 1.52 | 1.52 |
Abbe number | 56 | 55 |
Thickness | #1.5 (180 μm) | #1.5 (170 μm) |
Material | Microscopy plastic | D 263M Schott borosilicate glass |
Autofluorescence | Low | Low |
Transmission | Very high (even ultraviolet) | High (ultraviolet restrictions) |
Birefringence (DIC) | Low (DIC compatible*) | Low (DIC compatible*) |
Other aspects | ||
Surface modifications | ibiTreat – tissue culture treated Uncoated – hydrophobic | Only pure glass |
Protein coatings | Possible | Possible |
Gas permeable | Yes | No |
Material flexibility | High | Low |
Breakable | No | Yes |
Recommended for | All kinds of fluorescence microscopy | TIRF and single photon |