實(shí)驗(yàn)?zāi)康模?/strong>
多樣品組織研磨機(jī)研磨導(dǎo)兔子腿骨,提取DNA。
實(shí)驗(yàn)地點(diǎn):
首都醫(yī)科大學(xué)附屬友誼醫(yī)院
實(shí)驗(yàn)材料和器具
樣品:兔子腿骨
儀器:TISSUELYSER研磨機(jī)(上海凈信,TISSUELYSER-24)
(上海凈信,TISSUELYSER-24)
配件:不銹鋼研磨罐2個(gè)(上海凈信),
研磨珠(上海凈信)
研磨條件:冷凍干磨
實(shí)驗(yàn)步驟
1. 將凈信研磨罐置于液氮預(yù)冷備用
2.將兔子腿骨放在液氮中幾分鐘,然后剪成小塊備用。
3.將小段的腿骨放在研磨罐里,蓋好蓋子。
4.將裝好腿骨的研磨罐從液氮中取出裝入研磨儀,擰緊旋鈕。
4.設(shè)定好參數(shù),啟動(dòng)儀器至研磨程序結(jié)束后再次預(yù)冷模塊重復(fù)程序幾次。
(樣品實(shí)驗(yàn)前后對比)
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